Ultra-Fast High Temperature Liquid Chromatography

Chromatographers have long known that an increase in operating temperature can dramatically improve both the efficiency and speed of an HPLC separation. There are three main effects that happen at elevated temperature. Firstly, the viscosity of the mobile phase is decreased, so that higher flow rates are possible with existing equipment without increasing backpressure. Secondly, at higher temperature, the diffusion of analytes into and back out of the pores of a chromatographic material is faster, so the efficiency of the separation generally improves. Finally, at elevated temperature, the kinetics of the interactions between the analytes and the stationary phase are faster. This will lower the overall retention, but also often reduces or eliminates tailed peaks.

High Temperature Links:

Improving Throughput with High Temperature Presentation (PDF)

E-Newsletter Vol 1 - "Using Elevated Temperature to Improve HPLC Separations", May 2002

E-Newsletter Vol 5 - "High Temperature - The Future of HPLC!", May 2003

High Throughput - High Temperature LC/ Electrospray-MS Poster (PDF)

(Poster Courtesy of LCMS Limited, PO Box 27228, Raleigh, NC, USA 27611)

Reducing Run Time with Elevated Temperature


High Temperature Applications:

PDF Column(s) Compound Class Details
235 DB-C18 Parabens and Phenols Uracil, Phenol, Methyl Paraben, Ethyl Paraben, Propyl Paraben, Butyl Paraben, Heptyl Paraben
259 PBD Antihistamines Doxylamine, Methapyrilene, Chlorpheniramine, Triprolidine, Meclizine; Use of High Temperature
260 DB-C18 Barbiturates Barbital, Butabarbital, Pentobarbital, Carbromal, Secobarbital, Methohexital
261 PBD Benzodiazepines 7-aminoflunitrazepam, Clonazepam, Alprazolam, Nordiazepam, Diazepam, Medazepam
266 CARB Steroids/structurally similar Dihydroestradiol Sulfate, Dihydroequilin Sulfate, Equilin Sulfate, Estrone Sulfate, Equilenin Sulfate, Dihydroequilenin Sulfate
269 PBD Antihistamines Doxylamine, Methapyrilene, Chlorpheniramine, Triprolidine, Meclizine; Use of High Temperature
271 PS PAHs Napthalene, 1-Methylnapthalene, Acenapthylene, Acenapthene,  Fluorene, Decafluorobiphenyl, Phenanthrene,  Anthracene, Fluoranthene, Pyrene, p-terphenyl-d14, benzo(a)anthracene, benzo(b)fluoranthene, Benzo(a)pyrene, Dibenzo(a,h)anthracene, Benzo(g,h,i)perylene
276 PBD Antihypoglycemic Glyburide
279 DB-C18 Food/Cosmetics Nitrosamines
284 DB-C18 Steroids Epietiocholanolone, Etiocholanolone, Androsterone, Epiandrosterone

NOW AVAILABLE - Short ZirChrom Columns for UFHTLC

ZirChrom now offers short (2 cm) all-stainless steel pre-columns for ultra-fast high temperature reversed-phase liquid chromatography.  These columns are only sold in sets of two.  Mix and match ZirChrom chemistries if you like!  Contact ZirChrom technical support at 1-866-STABLE-1 for more details, or feel free to contact us online.


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