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In
this special edition of Z-APPS we have included six new application
notes. This research
highlights the unparalleled selectivity of our titanium dioxide and
zirconium dioxide particles (in bulk or in Glygen Lab-in-a-tip™
SPE tips) for phosphopeptide enrichment.
Technical
Bulletin #322
– Phosphopeptide
Enrichment using Sachtopore®-NP
Titanium Dioxide
– In this application note we report a rapid, highly
selective enrichment procedure utilizing 2,5-dihydroxybenzoic acid (DHB)
to enhance the selective enrichment of phosphorylated peptides on a
titanium dioxide micro-column. This unique technique dramatically
increases the selectivity, and thus sensitivity, of enrichment
purification of phosphorylated peptides from complex mixtures of non-phosphorylated
and phosphorylated peptides. (PDF
- Full Application Note)
Technical
Bulletin #323 – Phosphopeptide Enrichment Using
Titanium Dioxide & Zirconium Dioxide SPE Tips
– This application
note reports a rapid, highly selective enrichment procedure for
phosphopeptides utilizing titanium dioxide (TiO2) &
zirconium dioxide (ZrO2) SPE tips.
β-casein digest samples purified via NuTip™ SPE tips,
manufactured by Glygen Corporation (
Columbia
,
MD
), display exceptional signal to noise ratios for phosphopeptide
analysis and eliminate many difficulties present in traditional IMAC
methods. (PDF -
Full Application Note)
Technical
Bulletin #324 – Effect of Loading Buffer on
Phosphopeptide Enrichment using Zirconium Dioxide SPE Tips
– An investigation of
the effect of sample loading buffer on a rapid, highly selective
enrichment procedure for phosphopeptides utilizing zirconium dioxide
(ZrO2) SPE tips. For
α-casein digest samples enriched using ZrO2 NuTip™
SPE tips, manufactured by Glygen Corporation (Columbia, MD), a low pH
formic acid loading buffer enabled the most effective and specific
enrichment of phosphopeptides. (PDF
- Full Application Note)
Technical Bulletin #325 – Comparison
of Titanium Dioxide & Zirconium Dioxide SPE Tips for
Phosphopeptide Enrichment – A
critical comparison of zirconium dioxide (ZrO2) and
titanium dioxide (TiO2) SPE tips for rapid enrichment of
phosphopeptides. Although,
for the α-casein digest samples tested, either technique proves
more effective than traditional methods, interestingly, the ZrO2
tips enriched singly phosphorylated peptides in greater abundance. (PDF
- Full Application Note)
Technical Bulletin #326 – Selectivity
of Titanium Dioxide & Zirconium Dioxide SPE Tips for
Phosphopeptide Enrichment – This
application note compares and contrasts the selectivity of zirconium
dioxide (ZrO2) and titanium dioxide (TiO2) SPE
tips with varying concentrations of α-casein for rapid enrichment
of phosphopeptides. The
results demonstrate that phosphopeptide selectivity for α-casein
was not compromised when sample amount was decreased to 25 pmol.
Below 25 pmol, a smaller SPE tip (25 μg)
is required to obtain acceptable signal to noise ratios. (PDF
- Full Application Note)
To
place an order for Glygen’s Lab-in-a-tip™
SPE tips, or for more
information, please contact us at 1-866-STABLE-1 or visit http://www.zirchrom.com/glygen.asp.
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