ZAPPS Volume 16:  

Unique Selectivity of ZirChrom®-CARB for Difficult Separations: Orthogonal Selectivity, Structurally Similar Analytes, and Small Polar Molecules

In this special EAS edition of Z-APPS we have included three new application notes that feature separations on the ZirChrom®-CARB phase.  This research explores how the unique selectivity and stability of ZirChrom®-CARB can be employed to meet the toughest separation challenges.

ZirChrom will be exhibiting at the upcoming AAPS National Biotechnology Conference in San Diego.   To learn more about this cutting edge research visit us at our EAS booth #419 on May 1-2nd, 2017.

Orthogonal Selectivity: Rentention of 22 Solutes on ODS, ZirChrom®-PBD and  ZirChrom®-CARB - Comparison of retention of these 22 solutes shows the orthogonal selectivity of ZirChrom®-CARB phase when compared to both ODS and ZirChrom®-PBD. This unique selectivity allows separation of hard to resolve closely related compounds and impurities.   (PDF - Full Application Note)

Fast Methods for Structually Similar Compounds - Method development in reversed-phase liquid chromatography (RPLC) has traditionally been difficult for molecules which are geometric isomers or structurally very similar. In bonded-phase silicas, the partition mechanism responsible for retention in RPLC often does not offer adequate chemical selectivity for such compounds. ZirChrom®-CARB provides retention in RPLC through an adsorption mechanism which often times increases the chromatographic selectivity for these types of compounds and dramatically increases the chances of resolving pairs of these analytes. In addition to this enhanced chromatographic selectivity, ZirChrom®-CARB also offers increased chemical and stability of the stationary phase - pH 1-14 and temperatures up to 200°C.   (PDF - Full Application Note)

Fast Separation of Acrylamide Monomer from Acrylic Acid on ZirChrom®-CARB - Small polar molecules such as acrylamide and acrylic acid can be difficult if not impossible to separate on conventional reversed-phase bonded silica type phases using HPLC. Acrylamide historically has been analyzed using sample derivitization coupled with gas chromatography. The distinctive selectivity and increased retentiveness of the ZirChrom®-CARB phase, compared to bonded silica or polymeric phases, enables the baseline resolution of these small polar molecules  (PDF - Full Application Note)

Join Us! - ZirChrom Separations will be exhibiting at AAPS NBC 2017 on May 1st through May 2nd.  ZirChrom technical experts will be staffing the ZirChrom booth, booth #419, and would be happy to assist with your most difficult separations.

To place an order for our products, or for more information, please contact us at 1-866-STABLE-1 or .


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